Microscopy, oil immersion is a technique used to increase the resolving power of a microscope. This is achieved by immersing both the…
Microscopy, oil immersion is a technique used to increase the resolving power of a microscope.
This is achieved by immersing both the objective lens and the specimen in a transparent oil of high refractive index,
thereby increasing the numerical aperture of the objective lens
Oil Immersion Microscopy increases the refractive index of a specimen when used properly. With limited disadvantages, slides prepared with oil immersion techniques work best under higher magnification where oils increase refraction despite short focal lengths.
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