BROMOPHENOL BLUE ACS GRADE excellent quality manufacturer, exporter and supplier. A tracking dye for nucleic acid and protein gels. pH indicator is (Yellow to Blue; range 3.0 - 4.6).
BROMOPHENOL BLUE ACS GRADE excellent quality manufacturer, exporter and supplier. A tracking dye for nucleic acid and protein gels. pH indicator is (Yellow to Blue; range 3.0 - 4.6). Bromophenol blue migrates at approximately the same rate as 300-500bp DNA in agarose gel and at the buffer front in protein polyacrylamide gels.
C.A.S. No.
115-39-9
M.F.
C19H10Br4O5S
M.W.
669.99gm/mole
Specifications:
Dye Content (by Spectrophotometry)
>95%
Transition Range
pH 3.0-4.6
Greenish Yellow-Blue
Solubility 0.1% (95% EtOH)
Clear solution
Absorption maximum (pH 3.0) ?1max
434-439nm
Absorption maximum (pH 4.6) ?2max
590-593nm
Absorptivity (A1%/1cm at pH 3.0 at ?1max)
350-385
Absorptivity (A1%/1cm at pH 4.6 at ?2max)
940-1000
Loss On Drying (110°C)
<1%
Bromophenol blue is a tracking dye for alkaline and neutral buffer systems. It is used as a tracking dye in DNA, RNA (agarose) and protein (polyacrylamide) gel electrophoresis.