Cell cycle analysis is important for cancer research. We present herein a novel method for accurate cell cycle analysis. This method analyzes the cell cycle by multiparameter flow cytometry based on simultaneously labeling the cell nuclear DNA, RNA, and phosphorylated mitotic nuclei protein, using Hoechst 33342, pyronin Y, and MPM-2-Cy5, respectively, and our results demonstrated that this method could effectively divide the cell cycle into G0, G1, S, G2, and M phases.

Quinoline is the most privileged scaffold found in many natural products, drugs, and pharmaceutical substances . It is an important chemical moiety frequently used for the design and synthesis of large spectra of derivatives. In recent times, the quinoline nucleus has attracted many chemists as well as biologists as it is one of the key building elements for the construction of many pharmacologically active substances and drugs. 

 S-Benzyl isothiouronium chloride was synthesized from thiourea and benzyl chloride in the presence of ethanol. The chemicals used were of AnalaR grade. The melting point of the recrystallized sample was found to be 148°C, which is coinciding with the available literature

Acyl chlorides are used to prepare acid anhydrides, amides and esters, by reacting acid chlorides with: a salt of a carboxylic acid, an amine, or an alcohol, respectively.

Potassium persulfate is the inorganic compound with the formula K2S2O8. Also known as potassium peroxydisulfate or KPS, it is a white solid that is sparingly soluble in cold water, but dissolves better in warm water.

Sodium Persulfate is a white crystalline powder. It is used as a bleaching agent for fats, textiles and hair, a battery depolarizer, and in emulsion polymerization

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Ponceau S is a anionic dye commonly used for the detection of electro- blotted proteins after an electrophoretic separation prior to Western blotting [12–14] or MALDI-MS analysis [15].Ponceau S Staining Solution is supplied as ready to use. This product is recommended for rapid and reversible protein staining on nitrocellulose or PVDF membranes. This staining technique is often utilized to confirm protein electrotransfer in Western blotting assays prior to antibody-based detection.

Propylene Carbonate is used as a physical solvent for removal of carbon dioxide (CO2) and hydrogen sulfide (H2S) in natural gas treating, oil pyrolysis gas, and synthetic ammonia conversion gas applications. It is also used as an oil solvent and as an extraction agent for olefins and aromatics.

Propylene carbonate is of low acute toxicity in experimental animal following a single oral administration; no data have been found for inhalation exposure.

Phenylmercuric acetate is an organomercury compound. This compound was formerly used as a preservative in paints, and as a disinfectant.

 as an herbicide and fungicide. as such, is mixed with organic solvent for the purpose of application. Phenylmercury acetate is an arylmercury compound and a member of benzenes.

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Quinaldine red (pronounced /ˈkwɪnəldiːn/, abbreviated QR) is a dark green–red or black solid that does not dissolve easily in water (it is partly miscible). In addition to being used as colored indicator, quinaldine red is also used as a fluorescence probe and an agent in bleaching.

Toluidine is used as an intermediate in the synthesis of the large-volume herbicides, metolachlor and acetochlor, in the manufacture of more than 90 dyes and pigments (e.g. acid-fast dyestuffs, azo pigment dyes, triarylmethane dyes, sulfur dyes, and indigo compounds), and as an intermediate for synthetic rubber .Very slightly soluble in water. P-TOLUIDINE neutralizes acids to form salts plus water in exothermic reactions.

A method for detecting multidrug-resistant Mycobacterium tuberculosis by using a reduction of resazurin is described. Eighty clinical isolates were evaluated against isoniazid and rifampin; results at 7 days were compared with those of the proportion method. Specificity and sensitivity were excellent. The method is simple, inexpensive, and rapid and might be used with other antituberculosis drugs.

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