Fast blue B is used for the determination of acetylcholinesterase activity. Acetylcholinesterase converts 1-naphthyl acetate to 1-naphthol, which reacts with fast blue B to form a purple-coloured diazonium dye. Similarly, fast blue B is also used for α- and β-glucosidase activity.

Store at room temperature. Incompatible with strong oxidizing agents.

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The last addition of EDTA then removes the metal ion from its

 complex with the indicator, producing a color change

EDTA salts are more soluble in water as the pH increases: the more EDTA there is in the salt form, the higher the pH of a water solution, and therefore, the higher the room temperature solubility.

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Ethyl acetate's major use is as a solvent and are present in varnishes, perfumes, paints, nail varnish remover etc. Ethyl Acetate LR grade and Ethyl Acetate AR grade are the most commonly and easily available grades. The risk of toxicity is low but with concentrated exposture one may experience drowsiness.

Ferric Chloride is also called Iron(III) Chloride. It is soluble in water and alcohol and is non-combustible. When dissolved in water, ferric chloride undergoes hydrolysis and gives off heat in an exothermic reaction.

Formaldehyde is a strong-smelling, colorless gas used in making building materials and many household products. It is used in pressed-wood products, such as particleboard, plywood, and fiberboard; glues and adhesives; permanent-press fabrics; paper product coatings; and certain insulation materials.

Formalin is a 40% solution of formaldehyde in water.

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 The EDTA Solution Preparation and Recipe

Prepare 800 mL of distilled water in a suitable container.

Add 11.7 g of EDTA to the solution.

Slowly adjust the pH to 8.0 with 2 M NaOH. 

(Unless using the salt, EDTA often requires extended time and basic

1.  add 100 g EDTA and begin stirring. Adjust pH as needed to 7.2-7.4 by cautiously adding drops of 10N NaOH (or pellets). Cover and stir until completely dissolved.

Oxidation/reduction (redox) indicators are colorimetric reagents which show a distinct color change at a specific electrode potential. These are all organic compounds exhibiting reversible redox reactions. Examples include anilinic acid, diphenylamine, eriogreen, m-cresol-indophenol, methylene blue, and Nile blue.

Eugenol, also called clove oil, is an aromatic oil extracted from cloves that is used widely as a flavoring for foods and teas and as an herbal oil used topically to treat toothache and more rarely to be taken orally to treat gastrointestinal and respiratory complaints.

Folic acid is known as vitamin B9 which has vital functions. Our body needs folic acid for the synthesis, repair, and methylation of DNA. Moreover, it acts as a cofactor in many vital biological reactions. Folate has an important role in cell division and it is especially needed during infancy and pregnancy.

Diisopropyl ether can be used in a wide variety of applications. Its most common use is as a

solvent as oil-based solutions dissolve in it.