TRIS BUFFER LR
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Tris buffers are widely used for DNA agarose electrophoresis. The two main buffers are TBE (Tris borate/EDTA) and TAE (Tris acetate/EDTA). Although there are some differences in the resolution of different forms of DNA and their mobility during electrophoresis, these Tris buffers can generally be used interchangeably.
Key facts
- Product
- TRIS BUFFER LR
- Part No.
- RXSOL-60-6605-778
- Category
- Lab Chemical
- Supplier
- Muscat Chemical, Barka, South Al Batinah, Oman
- Delivery
- Muscat, Sohar, Salalah, Duqm, Nizwa, Sur, Barka and Gulf ports
- Documents
- MSDS and TDS on request
- Pricing
- Quote on request — contact us or WhatsApp +968 77333599
Tris, or tris(hydroxymethyl) aminomethane, is a common biological buffer, used throughout the DNA extraction process. During extraction from any number of sources, DNA is pH sensitive. During cell lysis, removal of unwanted cellular components and precipitation, tris is used to maintain a stable pH.
Dosage
To acquire a buffering capacity it sould be in equilibrium with the corresponding acid which is the protonated form of Tris. Traditionally, protonation of Tris is accomplished by addition of HCl. pH of the solution decreases to the desired value and the resulting Tris-HCl solution is what we commonly call Tris buffer