Folin & Ciocalteu's phenol reagent is used to determine protein concentrations between 1 to 100 μg/mL. However, the reagent exhibits a linear response to protein concentration up to 1000 μg/mL when used according to the instructions for kit Product No. TP0200.
The Folin-Ciocalteu (F-C) Assay was developed in 1927 for the measurement of tyrosine (1). The reagent consists of a mixture of sodium molybdate, sodium tungstate and other reagents. Upon reaction with phenols, it produces a blue color which absorbs at 765 nm.
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