Add Gram's iodine for 1 minute- this is a mordant, or an agent that fixes the crystal violet to the bacterial cell wall. Rinse sample/slide with acetone or alcohol for ~3 seconds and rinse with a gentle stream of water. The alcohol will decolorize the sample if it is Gram negative, removing the crystal violet.
Exposing gram negative cells to the decolorizer dissolves the lipids in the cell walls, which allows the crystal violet-iodine complex to leach out of the cells. This allows the cells to subsequently be stained with safranin.
GRAMS IODINE FOR MICROSCOPICAL STAINING Supplier Oman Muscat Barka Salalah Sohar Ruwi Mutrah Nizwa Al Hamra Manah
Email:- mail@muscatchemical.com
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