In order to see the spots on the chromatogram, you will apply a solution of ninhydrin to the paper.
Ninhydrin will react with the amino acid to produce a purple compound. Silica gel will serve as the stationary phase in the thin layer chromatography procedures.
TCL is based on the principle of separation through adsorption type. The separation relies on the relative empathy of compounds towards the mobile phase and stationary phase.
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