Ponceau S is a anionic dye commonly used for the detection of electro- blotted proteins after an electrophoretic separation prior to Western blotting [12–14] or MALDI-MS analysis [15].Ponceau S Staining Solution is supplied as ready to use. This product is recommended for rapid and reversible protein staining on nitrocellulose or PVDF membranes. This staining technique is often utilized to confirm protein electrotransfer in Western blotting assays prior to antibody-based detection.
Ponceau S is a negative stain which binds to the positively charged amino groups of the protein. It also binds non-covalently to non-polar regions in the protein. (Note: Ponceau S is not suitable for use with nylon membranes.) Ponceau S Staining Solution contains 0.1% Ponceau S (w/v) and 5.0% acetic Acid (w/v).
PONCEAU S FOR ELECTROPHORESIS Supplier Oman Muscat Barka Salalah Sohar Ruwi Mutrah Nizwa Al Hamra Manah
Email:- mail@muscatchemical.com