The thymidine analog 5-bromo-2′-deoxyuridine (BrdU) is used to label dividing cells. BrdU is injected intraperitoneally and incorporates into DNA being replicated where it will remain for the life of the cell.
Radioactive labelling techniques are of major importance in the study of DNA synthesis in bacteria. In order to label DNA in Escherichia coli either radioactive thymine or thymidine is generally used because these compounds are specifically incorporated into DNA.
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