Tricine is a commonly used electrophoresis buffer and is also used for the resuspension of cell pellets. It has a lower negative charge than glycine, allowing it to migrate faster. In addition, its high ionic strength causes more ion movement and less protein movement.
Tricine is an organic compound that is used in buffer solutions. The name tricine comes from tris and glycine, from which it was derived. It is a white crystalline powder that is moderately soluble in water. It is a zwitterionic amino acid that has a pKa1 value of 2.3 at 25 °C, while its pKa2 at 20 °C is 8.15.
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