Triethylamine is used to mask free silanol groups on the HPLC column and therefore it improves peak shape of the analytes. Moreover it can be used to increase/adjust the pH of the mobile phase and serve both the purpose of pH adjustment and peak shape improvement.
The acidity or basicity of the mobile phase can have a significant influence on separation. Acetic acid and triethylamine, as two traditional mobile phase additives in reverse phase HPLC, were employed to establish acidic or alkaline conditions, respectively.
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