Trifluoroacetic acid is an organofluorine compound with the chemical formula CF₃CO₂H. It is a structural analogue of acetic acid with all three of the acetyl group's hydrogen atoms replaced by fluorine atoms and is a colorless liquid with a vinegar-like odor.
Trifluoroacetic acid (TFA) is commonly used to release synthesized peptides from solid-phase resins. TFA or acetate is also used during the reversed-phase HPLC purification of peptides. The acids at low concentration are helpful for peptide solubility and HPLC separation.
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