In HPLC analysis, water is essential for use in preparing mobile phases and samples. If impurities are present in a mobile phase, however, they can increase background signals that can increase baseline noise, drift, or cause other phenomena, such as negative peaks or unknown peaks during gradient elution.
HPLC is a technique used to separate, identify, and quantify each component in a mixture. Using a pump, a solvent is pressurized through a column to separate and detect these components. Water is used as the solvent to prepare the standards for the mobile phase of HPLC. With HPLC, water purity is critical.
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