N,N,N′,N′-tetramethyl-p-phenylenediamine dihydrochloride (TMPD) is an oxidizable compound that serves as a reducing co-substrate for heme peroxidases. It is also used as a test reagent in microbiology for the classification of cytochrome oxidase positive aerobic microorganisms.
The term "Wurster's blue" is often reserved for the radical cation, the colorless diamine being called tetramethylphenylenediamine (TMPD). The midpoint potential for titration of the first electron is given as 0.276 V vs NHE, and this transition is useful in potentiometric titrations as both a redox mediator and indicator. The two electron-oxidized form (di-iminium) is unstable in aqueous solutions,[1] therefore highly oxidizing conditions should be avoided in titrations relying on TMPD, or reached only during the final stage of the titration.
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